Terstappen L W, Huang S
Becton Dickinson Immunocytometry Systems, San Jose, California 95131.
Blood Cells. 1994;20(1):45-61; discussion 61-3.
In this study we define hematopoietic stem cells (HSCs) as a population of cells that, when sorted as single cells, gives rise to both myeloid as well as lymphoid progeny. We sorted single cells from four populations of CD34+ cells from fetal bone marrow: (1) CD38- HLA-DR-, (2) CD38- HLA-DR+, (3) CD38+ HLA-DR-, and (4) CD38+ HLA-DR+ into liquid culture media supplemented with interleukin-3 (IL-3) IL-6, stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor, erythropoietin, basic fibroblast growth factor (bFGF) and insuline-like growth factor (IGF-1). The HSCs were found in the cell populations lacking CD38, the plating efficiency was highest in the CD34+ CD38- HLA-DR+ cell population (48% n = 12); however, only a small proportion of the CD34+ CD38- HLA-DR+ cells showed both lymphoid and myeloid growth potential. When the identical cell populations were sorted into liquid culture media supplemented with bFGF and IGF-1, cell growth was noted from only 1%-5% of the sorted CD34+ CD38- HLA-DR- cells. The cells have the potential to grow and differentiate in vitro to form complex structures that recapitulate normal bone formation. Serial passages of the progeny from these cultures resulted in the formation of similar structures.
在本研究中，我们将造血干细胞（HSCs）定义为这样一群细胞：当作为单个细胞进行分选时，它们能够产生髓系和淋巴系子代细胞。我们从胎儿骨髓的四个CD34+细胞群体中分选单个细胞：（1）CD38-HLA-DR-，（2）CD38-HLA-DR+，（3）CD38+HLA-DR-，以及（4）CD38+HLA-DR+，并将其接种到添加了白细胞介素-3（IL-3）、IL-6、干细胞因子（SCF）、粒细胞-巨噬细胞集落刺激因子、促红细胞生成素、碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子（IGF-1）的液体培养基中。造血干细胞存在于缺乏CD38的细胞群体中，接种效率在CD34+CD38-HLA-DR+细胞群体中最高（48%，n = 12）；然而，只有一小部分CD34+CD38-HLA-DR+细胞显示出淋巴系和髓系生长潜能。当将相同的细胞群体分选到添加了bFGF和IGF-1的液体培养基中时，只有1%-5%的分选CD34+CD38-HLA-DR-细胞出现细胞生长。这些细胞具有在体外生长和分化以形成模拟正常骨形成的复杂结构的潜能。这些培养物子代的连续传代导致形成相似的结构。
