Ricciardolo F L, Nadel J A, Bertrand C, Yamawaki I, Chan B, Geppetti P
Cardiovascular Research Institute, University of California, San Francisco.
Eur J Pharmacol. 1994 Aug 11;261(1-2):127-32. doi: 10.1016/0014-2999(94)90310-7.
The plasma extravasation evoked by instillation of 5% ovalbumin in the nasal mucosa of sensitized guinea-pigs was potentiated by the neutral endopeptidase inhibitor, phosphoramidon, and was reduced by the tachykinin NK1 receptor antagonist, CP-96,345. The bradykinin B2 receptor antagonist, HOE 140, also reduced the plasma extravasation evoked by the antigen. The combination of HOE 140 and CP-96,345 did not increase further the inhibition caused by HOE 140 alone. Plasma extravasation evoked by instillation of capsaicin was abolished by CP-96,345. HOE 140 blocked and CP-96,345 markedly reduced plasma extravasation caused by instillation of bradykinin. Plasma extravasation evoked by instillation of substance P was not affected by HOE 140. We conclude that antigen challenge causes plasma extravasation in the nasal mucosa of sensitized guinea-pigs, an effect that is due in part to the release of tachykinins from sensory nerve endings. Our evidence suggests that tachykinin release in response to antigen is provoked mainly by the release of kinins.
在致敏豚鼠的鼻黏膜中，通过滴注5%卵清蛋白诱发的血浆外渗，可被中性内肽酶抑制剂磷酰胺增强，并被速激肽NK1受体拮抗剂CP-96,345降低。缓激肽B2受体拮抗剂HOE 140也可降低抗原诱发的血浆外渗。HOE 140与CP-96,345联合使用并未进一步增强单独使用HOE 140所产生的抑制作用。滴注辣椒素诱发的血浆外渗可被CP-96,345消除。HOE 140可阻断、CP-96,345可显著降低滴注缓激肽所引起的血浆外渗。滴注P物质诱发的血浆外渗不受HOE 140影响。我们得出结论，抗原激发可导致致敏豚鼠鼻黏膜出现血浆外渗，这种效应部分归因于感觉神经末梢释放速激肽。我们的证据表明，抗原刺激引起的速激肽释放主要是由激肽释放所引发的。
