Factors affecting intra and extracellular phospholipase A production by Salmonella typhi.

The effect of various physico-chemical factors on the production of intra and extracellular phospholipase A by Salmonella typhi was investigated. Maximum intracellular enzyme levels were observed when cells were grown in brain heart infusion broth, after 16h of incubation at 37 degrees C. Highest level of extracellular phospholipase A was also seen in the same medium (pH 7.0), but after 24 h of incubation at 37 degrees C. Agitation during incubation enhanced the enzyme synthesis. Addition of surfactants to the growth media significantly decreased both intra and extracellular phospholipase A production.