Pauwels O, Kiss R
Laboratory of Histology, Faculty of Medicine, Free University of Brussels, Belgium.
Anal Cell Pathol. 1994 Oct;7(3):235-50.
It has previously been demonstrated that the cell nuclei from 1 mouse mammary cancer and 2 neoplastic human bladder cell lines displayed common morphonuclear characteristics when they were made resistant to different anti-neoplastic agents. In the present work this experiment was repeated with a greater number of cell lines from different kinds of tumours. The nuclei from the sensitive and resistant cells were submitted to Feulgen staining and studied by means of computerized nuclear image analysis. This methodology made it possible to characterize the cell nuclei by means of 15 parameters including 1 geometric, 2 densitometric and 12 others quantitatively describing the chromatin pattern. The results showed that the morphonuclear feature of the cell lines significantly varied according to the extent of the resistance. The majority of the observations showed that the direction of the variations seemed to be a function of the origin of the tissue. On the basis of the experimental results reported here, we think that it would be possible to establish a model for the purpose of monitoring the effectiveness of anticancer treatment from a clinical point of view.
先前已经证明，来自1个小鼠乳腺癌细胞系和2个人类膀胱癌细胞系的细胞核在对不同的抗肿瘤药物产生抗性时表现出共同的形态核特征。在本研究中，使用更多来自不同类型肿瘤的细胞系重复了该实验。对敏感细胞和抗性细胞的细胞核进行福尔根染色，并通过计算机化核图像分析进行研究。这种方法使得通过15个参数来表征细胞核成为可能，其中包括1个几何参数、2个光密度参数和12个其他定量描述染色质模式的参数。结果表明，细胞系的形态核特征根据抗性程度有显著差异。大多数观察结果表明，变化的方向似乎是组织来源的函数。基于此处报道的实验结果，我们认为从临床角度建立一个用于监测抗癌治疗效果的模型是可能的。
