苏云金芽孢杆菌菌株及亚种与近缘物种的16S至23S核糖体基因间隔序列的比较分析。
Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species.
作者信息
Bourque S N, Valero J R, Lavoie M C, Levesque R C
机构信息
Natural Resources Canada, Canadian Forest Service--Quebec Region, Sainte-Foy.
出版信息
Appl Environ Microbiol. 1995 Apr;61(4):1623-6. doi: 10.1128/aem.61.4.1623-1626.1995.
Abstract
Bacillus thuringiensis spacer regions between the 16S and 23S rRNAs were amplified with conserved primers, designated 19-mer and 23-mer primers. A spacer region of 144 bp was determined for all of 6 B. thuringiensis strains, 7 B. thuringiensis subspecies, and 11 B. thuringiensis field isolates, as well as for the closely related species Bacillus cereus and Bacillus anthracis. Computer analysis and alignment of nucleotide sequences identified three mutations and one deletion in the intergenic spacer region (ISR) of B. thuringiensis subsp. kurstaki HD-1 when compared with ISR sequences from other subspecies. The same differences were identified between the ISR of B. thuringiensis strains and the ISR of B. cereus and B. anthracis. These minor differences do not seem to be sufficient to allow the design of a species-specific oligonucleotide probe.
摘要
使用名为19聚体和23聚体引物的保守引物扩增苏云金芽孢杆菌16S和23S rRNA之间的间隔区。测定了6株苏云金芽孢杆菌菌株、7个苏云金芽孢杆菌亚种、11个苏云金芽孢杆菌田间分离株以及密切相关的蜡样芽孢杆菌和炭疽芽孢杆菌的144 bp间隔区。核苷酸序列的计算机分析和比对确定，与其他亚种的间隔区序列相比，苏云金芽孢杆菌库尔斯塔克亚种HD-1的基因间隔区（ISR）存在三个突变和一个缺失。苏云金芽孢杆菌菌株的ISR与蜡样芽孢杆菌和炭疽芽孢杆菌的ISR之间也存在相同差异。这些微小差异似乎不足以设计出物种特异性寡核苷酸探针。
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