熊去氧胆酸对胰腺外分泌的刺激作用：一项体外研究

Stimulatory effect of ursodeoxycholate on pancreatic exocrine secretion in an in vitro study.

作者信息

Shinozaki H, Funakoshi A, Miyasaka K, Kitani K

机构信息

Social Insurance Inatsuki Hospital, Fukuoka, Japan.

出版信息

Pancreas. 1995 Apr;10(3):246-50. doi: 10.1097/00006676-199504000-00005.

DOI:10.1097/00006676-199504000-00005

PMID:7542768

Abstract

Ursodeoxycholate (UDCA; 300 microM) significantly stimulates amylase secretion from isolated rat pancreatic acini but not Ca2+ mobilization. Removal of extracellular Ca2+ completely abolished the UDCA-stimulated secretory response. Ca ionophore (A23187) potentiated the response. However, staurosporine, a protein kinase C inhibitor, did not affect the UDCA-stimulated amylase secretion. These results indicated that UDCA directly acted on the pancreatic acini and stimulated amylase secretion by the mechanism of stimulation of influx of extracellular Ca2+, but not through inositol [1,4,5] triphosphate and diacylglycerol formation or Ca2+ mobilization.

摘要

熊去氧胆酸（UDCA；300微摩尔）能显著刺激分离的大鼠胰腺腺泡分泌淀粉酶，但对钙离子动员无影响。去除细胞外钙离子可完全消除UDCA刺激的分泌反应。钙离子载体（A23187）可增强该反应。然而，蛋白激酶C抑制剂星形孢菌素并不影响UDCA刺激的淀粉酶分泌。这些结果表明，UDCA直接作用于胰腺腺泡，通过刺激细胞外钙离子内流的机制来刺激淀粉酶分泌，而非通过肌醇[1,4,5]三磷酸和二酰基甘油的形成或钙离子动员。