叙利亚仓鼠脑池内R型颗粒的生物学和分子研究。
Biological and molecular studies on Syrian hamster intracisternal R-type particles.
作者信息
Lesser J, Bittoun P, Emanoil-Ravier R, Peries J
机构信息
UPR A0043, CNRS Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.
出版信息
Arch Virol. 1995;140(1):95-109. doi: 10.1007/BF01309726.
Retrovirus-like intracisternal R-type particles (IRP) are structures present in Syrian hamster (Mesocricetus auratus) cells cultured in vitro where they appear either spontaneously or under chemical induction conditions. We have tested several chemical inducers and ten different cell lines, looking for the best IRP induction conditions. BHK21 cl. 13 showed the highest inducibility one day after a 24 h treatment with 1 microgram/ml of 5-aza-2'-deoxycytidine. Using detergent treatments and sucrose gradients, we obtained semi-purified IRP cores. A 7.2 kb RNA associated with the core fraction was revealed by hybridization with total Syrian hamster genomic DNA, but not with Syrian hamster intracisternal A particle (IAP) specific probes. This suggests that the IRP genes are distinct from IAP ones.
逆转录病毒样的核内R型颗粒（IRP）是存在于体外培养的叙利亚仓鼠（金仓鼠）细胞中的结构，它们在这些细胞中自发出现或在化学诱导条件下出现。我们测试了几种化学诱导剂和十种不同的细胞系，以寻找最佳的IRP诱导条件。在用1微克/毫升的5-氮杂-2'-脱氧胞苷处理24小时后的一天，BHK21 cl. 13显示出最高的诱导性。通过去污剂处理和蔗糖梯度，我们获得了半纯化的IRP核心。通过与叙利亚仓鼠全基因组DNA杂交，而不是与叙利亚仓鼠核内A颗粒（IAP）特异性探针杂交，揭示了与核心部分相关的7.2 kb RNA。这表明IRP基因与IAP基因不同。
Zotero 插件