Kordash T R, Freshwater L L, Amend M J
Miles Inc., Allergy Products, Spokane, WA 99207, USA.
Ann Allergy Asthma Immunol. 1995 Aug;75(2):101-6.
Allergenic extracts are standardized by using the ID50EAL (Intradermal Dilution for 50 mm sum of Erythema Determines the Allergy Unit) skin test technique to assign allergy units to reference preparations. When new batches of extracts are manufactured, they are compared with the reference by RAST inhibition or other in vitro techniques. This study was designed to determine whether basophil histamine release might be an additional useful in vitro test for the standardization of allergenic extracts.
Basophil histamine release, skin tests, and RAST were compared using several different allergenic extracts at many different strengths. Allergy units were calculated using skin test results and basophil histamine release.
Basophil histamine release, skin tests, and RAST correlated well (P < .01). Basophil histamine release-derived allergy units and skin test-derived allergy units were highly correlated (P < .01).
Basophil histamine release appears to be a useful tool for the standardization of allergenic extracts.
变应原提取物通过使用ID50EAL（50mm红斑总和的皮内稀释法确定过敏单位）皮肤试验技术进行标准化，以给参考制剂分配过敏单位。当生产新批次的提取物时，通过放射性变应原吸附试验抑制或其他体外技术将它们与参考制剂进行比较。本研究旨在确定嗜碱性粒细胞组胺释放是否可能是变应原提取物标准化的另一种有用的体外试验。
使用几种不同强度的变应原提取物对嗜碱性粒细胞组胺释放、皮肤试验和放射性变应原吸附试验进行比较。使用皮肤试验结果和嗜碱性粒细胞组胺释放计算过敏单位。
嗜碱性粒细胞组胺释放、皮肤试验和放射性变应原吸附试验相关性良好（P <.01）。嗜碱性粒细胞组胺释放衍生的过敏单位与皮肤试验衍生的过敏单位高度相关（P <.01）。
嗜碱性粒细胞组胺释放似乎是变应原提取物标准化的一种有用工具。
