通过嗜碱性粒细胞组胺释放对变应原提取物进行标准化。

Standardization of allergenic extracts by basophil histamine release.

作者信息

Kordash T R, Freshwater L L, Amend M J

机构信息

Miles Inc., Allergy Products, Spokane, WA 99207, USA.

出版信息

Ann Allergy Asthma Immunol. 1995 Aug;75(2):101-6.

PMID:7544227

Abstract

BACKGROUND

Allergenic extracts are standardized by using the ID50EAL (Intradermal Dilution for 50 mm sum of Erythema Determines the Allergy Unit) skin test technique to assign allergy units to reference preparations. When new batches of extracts are manufactured, they are compared with the reference by RAST inhibition or other in vitro techniques. This study was designed to determine whether basophil histamine release might be an additional useful in vitro test for the standardization of allergenic extracts.

METHODS

Basophil histamine release, skin tests, and RAST were compared using several different allergenic extracts at many different strengths. Allergy units were calculated using skin test results and basophil histamine release.

RESULTS

Basophil histamine release, skin tests, and RAST correlated well (P < .01). Basophil histamine release-derived allergy units and skin test-derived allergy units were highly correlated (P < .01).

CONCLUSION

Basophil histamine release appears to be a useful tool for the standardization of allergenic extracts.

摘要

背景

变应原提取物通过使用ID50EAL（50mm红斑总和的皮内稀释法确定过敏单位）皮肤试验技术进行标准化，以给参考制剂分配过敏单位。当生产新批次的提取物时，通过放射性变应原吸附试验抑制或其他体外技术将它们与参考制剂进行比较。本研究旨在确定嗜碱性粒细胞组胺释放是否可能是变应原提取物标准化的另一种有用的体外试验。