Adenohypophysial histophysiology of gonadectomized, fatectomized and steroid treated male frogs, Rana hexadactyla (Lesson).

Cytology of the pars distalis of unilaterally and bilaterally gonadectomized Rana hexadactyla kept for a period of 60, 75, 120, 150 and 180 days and those treated with testosterone (25 micrograms) and oestradiol (25 micrograms) was studied. Similarly adenohypophysial cytomorphology in unilaterally and bilaterally fatectomized animals and/or treated with aqueous fat body extract was also investigated. The results indicate the B2 and B3 cells of pars distalis are responsible for the gametogenetic activity. A1 and B2 cells may contain some fat mobilizing factor (FMF) for stimulating spermatogenesis.