Bonartseva G A, Shemakhanova N M
Biol Bull Acad Sci USSR. 1978 Sep-Oct;5(5):628-33.
The dependence of the nitrogenase activity of Rhizobium meliloti on the strain peculiarities of the cultures, the composition of the media used, and the metabolites of legume tissue cultures was demonstrated by the acetylene method. The nitrogenase activity is significantly higher in R. vigna than in R. meliloti, under the same experimental conditions. Enrichment of the Murashige-Skoog medium with arabinose (25 mM), succinate (25 mM), glutamine (2 mM nitrogen), and yeast extract (0.1%) substantially stimulated the nitrogenase activity of a pure culture of R. vigna. The maximum nitrogenase activity on this medium was noted when metabolites of sweet clover tissue were introduced.
通过乙炔法证明了苜蓿根瘤菌的固氮酶活性依赖于培养物的菌株特性、所用培养基的组成以及豆科植物组织培养物的代谢产物。在相同实验条件下，豇豆根瘤菌的固氮酶活性显著高于苜蓿根瘤菌。在Murashige-Skoog培养基中添加阿拉伯糖（25 mM）、琥珀酸盐（25 mM）、谷氨酰胺（2 mM氮）和酵母提取物（0.1%）可显著刺激豇豆根瘤菌纯培养物的固氮酶活性。当引入草木犀组织的代谢产物时，在该培养基上观察到了最大固氮酶活性。
