Rabinowitz S S, Lucas J J, Gootman P M
Children's Medical Center of Brooklyn, Division of Gastroenterology and Nutrition, NY, USA.
Cell Mol Biol Res. 1995;41(1):73-80.
The distribution of the enzyme synthesizing nitric oxide (NO) has been characterized in several mammalian enteric nervous systems. Two methods, immunohistochemical staining, employing anti-nitric oxide synthase antibodies, and histochemical localization of NADPH-diaphorase (NADPH-D), have given the same results. On the other hand, few studies have investigated nitric oxide synthase (NOS) in the gastrointestinal mucosa. Our study demonstrated the presence and distribution of the enzyme, NADPH-D, throughout all layers of the neonatal piglet intestinal tract. In the neonatal piglet, NADPH-D activity was found in nerve fibers parallel to the circular and to the longitudinal muscles and in the ganglion cells of Auerbach's plexus. However, the majority of NADPH-D activity was localized to the mucosa. Furthermore, the most intense activity in the mucosa was observed in villous epithelial cells. Other mucosal cells which were NADPH-D positive included the glandular epithelium and crypt cells. In addition, glandular epithelium in the deeper submucosa had very strong NADPH-D activity. Our results support the hypothesis that locally produced NO mediates physiological functions in the intestinal mucosa and submucosa.
一氧化氮（NO）合成酶在几种哺乳动物的肠神经系统中的分布已得到表征。两种方法，即使用抗一氧化氮合酶抗体的免疫组织化学染色和NADPH-黄递酶（NADPH-D）的组织化学定位，得出了相同的结果。另一方面，很少有研究调查胃肠道黏膜中的一氧化氮合酶（NOS）。我们的研究证明了该酶NADPH-D在新生仔猪肠道各层中的存在和分布。在新生仔猪中，在与环形肌和纵行肌平行的神经纤维以及奥尔巴赫神经丛的神经节细胞中发现了NADPH-D活性。然而，大部分NADPH-D活性定位于黏膜。此外，在绒毛上皮细胞中观察到黏膜中最强烈的活性。其他NADPH-D阳性的黏膜细胞包括腺上皮和隐窝细胞。此外，较深黏膜下层的腺上皮具有非常强的NADPH-D活性。我们的结果支持这样的假说，即局部产生的NO介导肠黏膜和黏膜下层的生理功能。
