Morita Y, Clemens M G, Bulkley G B
Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-4685, USA.
Shock. 1995 Jul;4(1):41-4. doi: 10.1097/00024382-199507000-00006.
We investigated the role of reactive oxygen metabolites (ROMs) as potential mediators of delayed "spontaneous" leukocyte adhesion to rat mesenteric venules after the manipulation for in vivo microscopy. Rats were anesthetized via tail vein and prepared for intravital microscopic viewing of a segment of mesenteric venule. Leukocyte rolling, adhesion, and emigration were quantitated every 30 min for 3 h. Intravital observation immediately after routine gentle manipulation revealed a substantial leukocyte rolling and a small number of adherent and emigrated leukocytes. This rolling leukocyte flux then declined to a minimal level for 60 min. The number of adherent and emigrated leukocytes did not change during the initial 90 min. After 120 min, the flux of rolling leukocytes, and adherent and emigrated leukocyte number began to increase and reached significant levels at 180 min. To determine the possible role of ROMs in this "spontaneous" adhesion, rats were treated continuously with superoxide dismutase (SOD) plus catalase given intravenously over 3 h. Rolling leukocyte flux after 120 min was significantly attenuated by SOD plus catalase. SOD + catalase also significantly inhibited delayed leukocyte adhesion and emigration. Allopurinol substantially inhibited xanthine oxidase activity but had no significant effects on the above parameters of neutrophil dynamics. These findings suggest that ROMs, probably derived from a source other than xanthine oxidase, constitute important mediators of "spontaneous" leukocyte adhesion in rat mesenteric venules.
我们研究了活性氧代谢产物（ROMs）在体内显微镜操作后，作为大鼠肠系膜小静脉延迟“自发性”白细胞黏附潜在介质的作用。通过尾静脉对大鼠进行麻醉，并准备好对一段肠系膜小静脉进行活体显微镜观察。每30分钟对白细胞滚动、黏附和游出情况进行定量，持续3小时。常规轻柔操作后立即进行的活体观察显示，有大量白细胞滚动，并有少量黏附和游出的白细胞。随后这种滚动的白细胞通量下降至最低水平并持续60分钟。在最初的90分钟内，黏附和游出的白细胞数量没有变化。120分钟后，滚动白细胞的通量以及黏附和游出的白细胞数量开始增加，并在180分钟时达到显著水平。为了确定ROMs在这种“自发性”黏附中的可能作用，对大鼠静脉内连续3小时给予超氧化物歧化酶（SOD）加过氧化氢酶进行治疗。120分钟后的滚动白细胞通量被SOD加过氧化氢酶显著减弱。SOD + 过氧化氢酶也显著抑制了延迟的白细胞黏附和游出。别嘌呤醇显著抑制了黄嘌呤氧化酶的活性，但对上述中性粒细胞动力学参数没有显著影响。这些发现表明，ROMs可能来源于黄嘌呤氧化酶以外的其他来源，是大鼠肠系膜小静脉中“自发性”白细胞黏附的重要介质。
