Gemmell E, Polak B, Reinhardt R A, Eccleston J, Seymour G J
Immunopathology Laboratory, Department of Dentistry, The University of Queensland, Australia.
Oral Dis. 1995 Jun;1(2):63-9. doi: 10.1111/j.1601-0825.1995.tb00161.x.
Porphyromonas gingivalis demonstrates a strong association with adult periodontitis although some individuals with the infection do not experience attachment loss. Therefore differences in the immune response to this organism may be of importance to the outcome of the disease.
The aim of this study was to determine whether P. gingivalis positive subjects with and without periodontal breakdown, reacted differently to P. gingivalis antigens as assessed by the pattern of serum antibody reactivity.
Two highly defined groups of subjects were chosen for this study. Both demonstrated P. gingivalis in their plaque and both had responded to P. gingivalis as shown by the presence of serum antibodies. The two groups differed only in their apparent clinical susceptibility to periodontal breakdown. Western blots of P. gingivalis membrane antigens were probed with sera from the two groups to determine their reactivity to specific antigens.
Analysis of the immunoblots showed that there were no differences in either the total numbers of bands, or bands recognized by the majority of subjects in the gingivitis and adult periodontitis groups. There were however, four bands recognized by the majority of the gingivitis group and not by the majority of the adult periodontitis group, there being a significant difference (P = 0.03) in the recognition of the 91.4-kDa antigen band. A further five antigens of lower molecular weight were seen by the majority of the adult periodontitis group and not by the majority of the gingivitis group. When sera were tested against purified P. gingivalis LPS, the results indicated that the five antigens seen by the majority of the adult periodontitis group had molecular weights which were in the range exhibited by the LPS antigens.
These results suggest that gingivitis and adult periodontitis subjects with P. gingivalis infection, may recognize different P. gingivalis antigens.
牙龈卟啉单胞菌与成人牙周炎有很强的关联，尽管一些感染该菌的个体并未出现附着丧失。因此，对这种微生物的免疫反应差异可能对疾病的转归很重要。
本研究的目的是确定有和没有牙周破坏的牙龈卟啉单胞菌阳性受试者，根据血清抗体反应模式评估，对牙龈卟啉单胞菌抗原的反应是否不同。
本研究选择了两组高度明确的受试者。两组受试者的菌斑中均检测到牙龈卟啉单胞菌，且血清抗体的存在表明他们均对牙龈卟啉单胞菌有反应。两组的区别仅在于其对牙周破坏的明显临床易感性。用两组受试者的血清检测牙龈卟啉单胞菌膜抗原的蛋白质免疫印迹，以确定他们对特定抗原的反应性。
免疫印迹分析表明，牙龈炎组和成人牙周炎组在条带总数或大多数受试者识别的条带方面均无差异。然而，有四条带被大多数牙龈炎组受试者识别，而未被大多数成人牙周炎组受试者识别，在对91.4 kDa抗原条带的识别上存在显著差异（P = 0.03）。大多数成人牙周炎组受试者还识别出另外五种分子量较低的抗原，而大多数牙龈炎组受试者未识别出。当用纯化的牙龈卟啉单胞菌脂多糖检测血清时，结果表明大多数成人牙周炎组受试者识别的五种抗原的分子量在脂多糖抗原显示的范围内。
这些结果表明感染牙龈卟啉单胞菌的牙龈炎和成人牙周炎受试者可能识别不同的牙龈卟啉单胞菌抗原。
