编码来自人类疟原虫恶性疟原虫的拓扑异构酶I的基因。

The gene encoding topoisomerase I from the human malaria parasite Plasmodium falciparum.

作者信息

Tosh K, Kilbey B

机构信息

Institute of Cell and Molecular Biology, University of Edinburgh, UK.

出版信息

Gene. 1995 Sep 22;163(1):151-4. doi: 10.1016/0378-1119(95)00376-h.

DOI:10.1016/0378-1119(95)00376-h

PMID:7557466

Abstract

Part of the topoisomerase I (TopoI)-encoding gene from Plasmodium falciparum (Pf) was isolated by PCR from cDNA using oligodeoxyribonucleotides modelled on the highly conserved regions of sequence from other species. The entire TopoI gene was obtained by screening a Pf K1 HindIII-EcoRI genomic library in lambda NM1149 with a random-labeled heterologous probe from the Saccharomyces cerevisiae TopoI gene. DNA sequence analysis revealed an open reading frame of 2520 nt encoding a deduced protein of 839 amino acids (aa) with no detectable introns. The Pf TopoI aa sequence has about 40% identity with most eukaryotic TopoI homologues. The gene is located as a single copy on chromosome 5 and Northern analysis identified a transcript of 3.8 kb.

摘要

利用基于其他物种序列高度保守区域设计的寡脱氧核糖核苷酸，通过聚合酶链反应（PCR）从恶性疟原虫（Pf）的cDNA中分离出部分拓扑异构酶I（TopoI）编码基因。通过用来自酿酒酵母TopoI基因的随机标记异源探针筛选λ NM1149中的Pf K1 HindIII-EcoRI基因组文库，获得了完整的TopoI基因。DNA序列分析揭示了一个2520 nt的开放阅读框，编码一个推导的含839个氨基酸（aa）的蛋白质，且未检测到内含子。Pf TopoI的氨基酸序列与大多数真核TopoI同源物具有约40%的同一性。该基因以单拷贝形式位于5号染色体上，Northern分析鉴定出一个3.8 kb的转录本。