Tarrade T, Crain S M
Dev Neurosci. 1978;1(3-4):119-32. doi: 10.1159/000112564.
Development and regional localization of patterned spontaneous bioelectric activities, recorded extracellularly, were studied in cultures of frontal sections of spinal cord and medulla, explanted from 13- to 15-day-old fetal mice. Spontaneous single- and multi-unit tonic and phasic spike discharges were recorded after 4-21 days in vitro. Stereotyped slow waves with positive or negative polarity, and durations up to several seconds, often occurred concomitant with spike burst discharges. Microelectrode mapping demonstrated regional localization of spontaneous activity patterns in these cross-sectional central nervous system explants, which were oriented so that dorsal and ventral borders could be regularly identified. Phasic discharges were widely distributed throughout the explants, whereas tonic discharges were located primarily in the dorsal region of the medulla; negative slow waves were generated in central and ventral regions of spinal cord and medulla explants, whereas positivities were located in the peripheral regions of the explants. Phasic discharges were often synchronous when recorded at two symmetrical regions of an oriented explant, as early as 4 days in vitro. The major developmental change in spontaneous activities of these explants was a progressive shortening of both burst and cycle durations of the phasic discharges.
对取自13至15日龄胎鼠的脊髓和延髓额部切片培养物中细胞外记录的模式化自发生物电活动的发育和区域定位进行了研究。体外培养4至21天后记录到自发的单单位和多单位紧张性和相位性放电。常伴随放电爆发出现极性为正或负、持续时间长达数秒的定型慢波。微电极测绘显示了这些中枢神经系统横断面外植体中自发活动模式的区域定位，这些外植体经过定向处理，以便能常规识别背侧和腹侧边界。相位性放电广泛分布于整个外植体，而紧张性放电主要位于延髓的背侧区域；负向慢波在脊髓和延髓外植体的中央和腹侧区域产生，而正向慢波位于外植体的周边区域。早在体外培养4天时，在定向外植体的两个对称区域记录时，相位性放电往往是同步的。这些外植体自发活动的主要发育变化是相位性放电的爆发和周期持续时间逐渐缩短。
