Streptavidin-reactive protein in Fasciola hepatica.

Streptavidin-binding protein was found in an extract of Fasciola hepatica, and the protein was analyzed by the inhibition assay using avidin or D-biotin, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using peroxidase-conjugated streptavidin. Inhibition assays revealed that the protein could bind to streptavidin by a mechanism similar to the avidin-biotin reaction, and the molecular size of the protein was estimated by SDS-PAGE to be 71.7 kDa.