Arnon A, Levi R
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Int Arch Allergy Immunol. 1995 Dec;108(4):321-6. doi: 10.1159/000237175.
Synthetic recombinant vaccines are expression vectors incorporating defined epitope(s) of microbial agents. They are prepared by inserting synthetic oligonucleotide(s) coding for previously identified relevant epitopes into the genome of a desired vector, using recombinant DNA technology. The results discussed indicate that immunization with such vaccines carrying viral epitopes may lead to protective immunity against viral agents. Oligonucleotides coding for three influenza epitopes stimulating B cells, T helper cells and cytotoxic lymphocytes were individually inserted into the flagellin gene of a Salmonella vaccine strain. Immunization of mice with the resultant recombinant bacteria or their isolated flagella induced a specific mucosal anti-influenza protective response. The most efficient vaccine consisted of all three recombinant flagella, administered intranasally. The protection elicited was cross-strain specific, long-lasting and efficient against a lethal viral challenge.
合成重组疫苗是包含微生物病原体特定表位的表达载体。它们是通过使用重组DNA技术将编码先前鉴定的相关表位的合成寡核苷酸插入所需载体的基因组中制备而成。所讨论的结果表明，用携带病毒表位的此类疫苗进行免疫可能会产生针对病毒病原体的保护性免疫。将编码刺激B细胞、辅助性T细胞和细胞毒性淋巴细胞的三种流感表位的寡核苷酸分别插入沙门氏菌疫苗株的鞭毛蛋白基因中。用所得重组细菌或其分离的鞭毛对小鼠进行免疫诱导了特异性黏膜抗流感保护性反应。最有效的疫苗由所有三种重组鞭毛组成，通过鼻内给药。所引发的保护具有交叉株特异性、持久且对致死性病毒攻击有效。
