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非洲象(Loxodonta africana)肠道黏液糖蛋白或黏蛋白的组织化学研究。

A histocytochemical study of mucus glycoproteins or mucins in the intestinal tract of the African elephant (Loxodonta africana).

作者信息

Kotzé S H, Coetzee H L

机构信息

Department of Anatomy, Faculty of Medicine, University of Pretoria, South Africa.

出版信息

Onderstepoort J Vet Res. 1994 Jun;61(2):177-81.

PMID:7596568
Abstract

The distribution of neutral mucins, sialomucins and sulphomucins was determined histochemically in the duodenum, jejunum, ileum and colon of the African elephant (Loxodonta africana). The techniques used were periodic acid-Schiff (PAS), alcian blue/periodic acid-Schiff (AB-PAS), high-iron-diamine/alcian blue (HID-AB), alcian blue at varying pH solutions and alcian blue at high temperature after methylation and saponification. Acid mucins appear to dominate neutral mucins, the latter decreasing toward the large intestine. Sulphomucins and sialomucins occurred in almost equal amounts throughout the intestinal tract, with a slight decrease of sialomucins toward the colon.

摘要

采用组织化学方法测定了非洲象(Loxodonta africana)十二指肠、空肠、回肠和结肠中中性黏蛋白、唾液酸黏蛋白和硫酸黏蛋白的分布情况。所采用的技术包括过碘酸希夫反应(PAS)、阿尔辛蓝/过碘酸希夫反应(AB-PAS)、高铁二胺/阿尔辛蓝(HID-AB)、在不同pH值溶液中的阿尔辛蓝以及甲基化和皂化后高温处理的阿尔辛蓝。酸性黏蛋白似乎比中性黏蛋白占优势,中性黏蛋白向大肠方向减少。硫酸黏蛋白和唾液酸黏蛋白在整个肠道中含量几乎相等,唾液酸黏蛋白向结肠方向略有减少。

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