Masella R, Pignatelli E, Marinelli T, Modesti D, Verna R, Cantafora A
Istituto Superiore di Sanità, Laboratory of Metabolism and Pathological Biochemistry, Rome, Italy.
Comp Biochem Physiol B Biochem Mol Biol. 1995 Jun;111(2):319-27. doi: 10.1016/0305-0491(94)00217-i.
Lipoprotein and liver lipids of spontaneously hyperlipidemic Yoshida rats were compared with those of normolipidemic Wistar animals for studying their age- and strain-related differences. Both strains showed an age-related increase in the total plasma cholesterol concentration. However, the Yoshida strain had a higher content of cholesteryl esters and triglycerides than the Wistar strain in both young and adult animals (2- and 8-month-old animals, respectively). The free cholesterol content was also higher, but only in the 8-month-old animals. Both strains showed an age-related increase in the proportion of HDL1 and a symmetrical decrease in both the HDL2 and HDL3 subfractions, but the variations were more evident in the Yoshida strain. The study of strain-related differences suggested that the spontaneous hypertriglyceridemia of the Yoshida strain was not only related to the higher amount and proportion of the VLDL fraction, but also to the higher content of triglycerides in the LDL fraction. The livers of Yoshida rats accumulated more triglycerides (with an age-related progression) than those of Wistar rats. The major lipid classes in the liver of Yoshida rats contained a significantly higher proportion of monounsaturated fatty acyls. Furthermore, this proportion showed an age-related increase in all the lipid classes, but in cholesteryl esters. This suggested that liver desaturases had a relevant role in the development of hyperlipidemia, and of its age-related variations, in the Yoshida strain.(ABSTRACT TRUNCATED AT 250 WORDS)
为研究自发性高脂血症吉田大鼠与血脂正常的Wistar大鼠脂蛋白和肝脏脂质的年龄及品系相关差异，对二者进行了比较。两个品系的血浆总胆固醇浓度均随年龄增长而升高。然而，在幼年和成年动物（分别为2月龄和8月龄）中，吉田品系的胆固醇酯和甘油三酯含量均高于Wistar品系。游离胆固醇含量也更高，但仅在8月龄动物中如此。两个品系的HDL1比例均随年龄增长而增加，HDL2和HDL3亚组分均呈对称下降，但吉田品系的变化更为明显。品系相关差异研究表明，吉田品系的自发性高甘油三酯血症不仅与VLDL组分的含量和比例较高有关，还与LDL组分中甘油三酯含量较高有关。吉田大鼠的肝脏比Wistar大鼠积累了更多的甘油三酯（且随年龄增长而加重）。吉田大鼠肝脏中的主要脂质类别含有显著更高比例的单不饱和脂肪酰基。此外，除胆固醇酯外，所有脂质类别中的这一比例均随年龄增长而增加。这表明肝脏去饱和酶在吉田品系高脂血症及其年龄相关变化的发展中起相关作用。（摘要截选至250词）
