Verrijzer C P, Chen J L, Yokomori K, Tjian R
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley 94720-3204, USA.
Cell. 1995 Jun 30;81(7):1115-25. doi: 10.1016/s0092-8674(05)80016-9.
The mechanisms that govern core promoter recognition and basal transcription efficiency remain poorly understood. Here, we have assessed the potential role of TAFs and the TFIID complex in directing basal promoter function. Reconstituted transcription reactions revealed the ability of TFIID versus TBP to discriminate between distinct core promoters. A comparison of different partial TBP-TAF assemblages established that a trimeric TBP-TAFII250-TAFII150 complex is minimally required for efficient utilization of the initiator and downstream promoter elements. Depending on the promoter structure, TAFs can increase or decrease the stability of TFIID-promoter interactions. These findings suggest that TAFs play a critical role in promoter selectivity and transcription regulation through direct contacts with core promoter elements.
目前，人们对核心启动子识别和基础转录效率的调控机制仍知之甚少。在此，我们评估了TAF和TFIID复合物在指导基础启动子功能方面的潜在作用。重组转录反应揭示了TFIID与TBP区分不同核心启动子的能力。对不同部分TBP-TAF组合的比较表明，三聚体TBP-TAFII250-TAFII150复合物是有效利用起始子和下游启动子元件所必需的最低要求。根据启动子结构，TAF可以增加或降低TFIID-启动子相互作用的稳定性。这些发现表明，TAF通过与核心启动子元件的直接接触，在启动子选择性和转录调控中发挥关键作用。
