Feussner I, Kühn H
Institut für Pflanzenbiochemie, Halle, Germany.
FEBS Lett. 1995 Jun 19;367(1):12-4. doi: 10.1016/0014-5793(95)00531-d.
The lipid body lipoxygenase of cucumber seedlings is at a high level expressed during the germinating process which is the stage of triglyceride mobilisation. This enzyme exhibits an unusual positional specificity which has not been described so far for any plant and animal lipoxygenase. The purified enzyme converts arachidonic acid to 15-S-hydroperoxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid (15S-HPETE), 12-S-hydroperoxy-5Z, 8Z, 10E, 14Z-eicosatetraenoic acid (12S-HPETE), and 8-S-hydroperoxy-5Z,9E,11Z,14Z-eicosatetraenoic acid (8S-HPETE) in a ratio of 76:4:20 with the corresponding R-isomers being only minor contaminants. Binding to the lipid bodies enhances the arachidonic acid dioxygenase activity more than 4-times and alters positional specificity of the enzyme in favour of 8-S-hydroperoxy-5Z,9E,11Z,14Z-eicosatetraenoic acid (8S-HPETE) formation.
黄瓜幼苗的脂质体脂氧合酶在萌发过程（即甘油三酯动员阶段）中高水平表达。这种酶表现出一种不同寻常的位置特异性，这在任何植物和动物脂氧合酶中均未被描述过。纯化后的该酶将花生四烯酸转化为15 - S - 氢过氧 - 5Z,8Z,11Z,13E - 二十碳四烯酸（15S - HPETE）、12 - S - 氢过氧 - 5Z,8Z,10E,14Z - 二十碳四烯酸（12S - HPETE）和8 - S - 氢过氧 - 5Z,9E,11Z,14Z - 二十碳四烯酸（8S - HPETE），比例为76:4:20，相应的R - 异构体仅为少量污染物。与脂质体结合使花生四烯酸双加氧酶活性增强4倍以上，并改变了该酶的位置特异性，有利于8 - S - 氢过氧 - 5Z,9E,11Z,14Z - 二十碳四烯酸（8S - HPETE）的形成。
