环磷酸腺苷在刺激诱发兔离体耳动脉释放3H-去甲肾上腺素中的作用。
Role of cyclic AMP in stimulation-evoked release of 3H-noradrenaline from rabbit isolated ear artery.
作者信息
Abrahamsen J, Nedergaard O A
机构信息
Department of Pharmacology, School of Medicine, Odense University, Denmark.
出版信息
Pharmacol Toxicol. 1995 Mar;76(3):185-9. doi: 10.1111/j.1600-0773.1995.tb00127.x.
The possible involvement of cyclic AMP in the stimulation-evoked 3H-overflow from rabbit isolated ear artery preloaded with 3H-noradrenaline was studied. Cyclic AMP (10(-5)-3 x 10(-4)M), 8-bromo-cyclic AMP (3 x 10(-4)M) and adenosine (10(-5)-3 x 10(-4)M) enhanced stimulation-evoked 3H-overflow. Dibutyryl-cyclic AMP (10(-5)-3 x 10(-4)M) had no effect. Theophylline (3 x 10(-5)M) and the phosphodiesterase inhibitor AH 21-132 (3 x 10(-5)M) did not alter the enhancement of 3H-overflow caused by either cyclic AMP or adenosine. Forskolin (3 x 10(-6)M) and the phosphodiesterase inhibitors ICI 63 197 (10(-4)M) and AH 21-132 (3 x 10(-6)-3 x 10(-5)M) increased stimulation-evoked 3H-overflow. Forskolin (10(-6)M) enhanced the effect of ICI 63 197 (3 x 10(-5)M) but it did not alter the effect of AH 21-132. It is concluded that cyclic AMP is involved in the stimulation-evoked release of noradrenaline from postganglionic sympathetic nerves in the rabbit ear artery.
研究了环磷酸腺苷（cAMP）在刺激诱发的、预先用3H-去甲肾上腺素加载的兔离体耳动脉中3H溢出过程中可能发挥的作用。环磷酸腺苷（10^(-5) - 3×10^(-4)M）、8-溴环磷酸腺苷（3×10^(-4)M）和腺苷（10^(-5) - 3×10^(-4)M）增强了刺激诱发的3H溢出。二丁酰环磷酸腺苷（10^(-5) - 3×10^(-4)M）没有作用。茶碱（3×10^(-5)M）和磷酸二酯酶抑制剂AH 21-132（3×10^(-5)M）并未改变由环磷酸腺苷或腺苷引起的3H溢出增强效应。福斯高林（3×10^(-6)M）以及磷酸二酯酶抑制剂ICI 63 197（10^(-4)M）和AH 21-132（3×10^(-6) - 3×10^(-5)M）增加了刺激诱发的3H溢出。福斯高林（10^(-6)M）增强了ICI 63 197（3×10^(-5)M）的效应，但未改变AH 21-132的效应。得出的结论是，环磷酸腺苷参与了兔耳动脉节后交感神经刺激诱发的去甲肾上腺素释放过程。
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