Schmader K, Henry S C, Rahija R J, Yu Y, Daley G G, Hamilton J D
Geriatric Research, Education, and Clinical Center, Durham VA Medical Center, North Carolina, USA.
J Infect Dis. 1995 Aug;172(2):531-4. doi: 10.1093/infdis/172.2.531.
The hypothesis that replication-competent mouse cytomegalovirus (MCMV) is detectable in severe combined immunodeficient (scid) mice after implantation of latently infected tissue was examined. Sections of latently infected salivary gland from 5 BALB/c mice were implanted into 20 C.B-17 scid mice. Recipient scid mice were sacrificed weekly for 5 weeks, and MCMV infection was detected in target organs using culture and DNA polymerase chain reaction (PCR). All donors were negative by standard culture but positive by DNA PCR. Replicating MCMV was recovered from 9 of 15 recipient scid mouse salivary gland, lung, liver, or spleen samples at postoperative weeks 2-5. No virus was recovered from recipient scid mice at weeks 1 or 12. Transplantation of latently infected tissues into scid mice resulted in rapid reactivation and dissemination of the virus. Further study of this model promises insight into the mechanisms of CMV latency and reactivation.
将潜伏感染的组织植入严重联合免疫缺陷（scid）小鼠后，是否能检测到具有复制能力的小鼠巨细胞病毒（MCMV）。从5只BALB/c小鼠的潜伏感染唾液腺切下组织块，植入20只C.B-17 scid小鼠体内。在5周的时间里，每周处死一批受体scid小鼠，通过培养和DNA聚合酶链反应（PCR）检测靶器官中的MCMV感染情况。所有供体经标准培养均为阴性，但DNA PCR检测呈阳性。在术后第2至5周，从15只受体scid小鼠的唾液腺、肺、肝或脾样本中的9个样本中分离出了具有复制能力的MCMV。在第1周或第12周，未从受体scid小鼠中分离到病毒。将潜伏感染的组织移植到scid小鼠体内会导致病毒迅速激活和扩散。对该模型的进一步研究有望深入了解CMV潜伏和激活的机制。
