体外培养的牛卵母细胞减数分裂成熟过程中的c-mos原癌基因产物
On the c-mos proto-oncogene product during meiotic maturation in bovine oocytes cultured in vitro.
作者信息
Tatemoto H, Terada T
机构信息
Department of Bioresources, Hiroshima Prefectural University, Japan.
出版信息
J Exp Zool. 1995 Jun 1;272(2):159-62. doi: 10.1002/jez.1402720210.
The present study was carried out using dot-blot Western analysis with pp39mos-specific polyclonal antibodies to examine the quantitative and qualitative changes of c-mos proto-oncogene product, Mos, during bovine oocyte maturation in vitro. Mos is present throughout meiotic maturation, is produced from around the onset of meiotic resumption, and is phosphorylated on germinal vesicle breakdown. These results indicate for the first time that the synthesis and phosphorylation of Mos during maturation culture play a key role in the accomplishment of meiosis in bovine oocytes.
本研究采用斑点印迹免疫印迹分析，使用pp39mos特异性多克隆抗体，检测体外培养的牛卵母细胞成熟过程中c-mos原癌基因产物Mos的定量和定性变化。Mos在整个减数分裂成熟过程中均有表达，从减数分裂恢复开始时左右产生，并在生发泡破裂时发生磷酸化。这些结果首次表明，成熟培养过程中Mos的合成和磷酸化在牛卵母细胞减数分裂的完成中起关键作用。
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