On the c-mos proto-oncogene product during meiotic maturation in bovine oocytes cultured in vitro.

The present study was carried out using dot-blot Western analysis with pp39mos-specific polyclonal antibodies to examine the quantitative and qualitative changes of c-mos proto-oncogene product, Mos, during bovine oocyte maturation in vitro. Mos is present throughout meiotic maturation, is produced from around the onset of meiotic resumption, and is phosphorylated on germinal vesicle breakdown. These results indicate for the first time that the synthesis and phosphorylation of Mos during maturation culture play a key role in the accomplishment of meiosis in bovine oocytes.