Mezrioui N, Oufdou K, Baleux B
Laboratoire de microbiologie, Département de biologie, Université Cadi Ayyad, Marrakech, Morocco.
Can J Microbiol. 1995 Jun;41(6):489-98. doi: 10.1139/m95-065.
We studied the spatial-temporal dynamics of non-O1 Vibrio cholerae numbers at a stabilization pond treatment plant. This bacterium's seasonal dynamics were the inverse of those of fecal coliforms, with high levels in hot periods and low levels in cold periods. Stabilization pond treatment did not significantly reduce non-O1 V. cholerae numbers between the system's inflow and outflow points. In contrast, fecal coliforms were reduced by 98.95% in hot periods and by 94.91% in cold periods. Significant rho coefficient values for the Spearman correlation between numbers of non-O1 V. cholerae and temperature and pH of 0.91 and 0.76 respectively, were found at the system's outflow point. An experimental study of the effects of pH, temperature, and sunlight on the survival of non-O1 V. cholerae and fecal coliforms confirmed the inverse behaviour of the two bacterial groups noted in the stabilization ponds. Alkaline pH values of 8 and 8.8 promoted the survival of non-O1 V. cholerae and inhibited that of Escherichia coli. Low temperatures (8 degrees C) prolonged E. coli survival (k = 0.002/h), while a temperature of 23 degrees C reduced it markedly (k = 0.022/h). Non-O1 V. cholerae did not survive as well as E. coli at 8 degrees C (k = 0.009/h). The effect of temperature on non-O1 V. cholerae appeared to be closely linked to nutrient levels. Non-O1 V. cholerae appeared to be less sensitive to sunlight than E. coli whose survival was markedly reduced particularly during summer periods. Non-O1 V. cholerae and E. coli did not behave in the same way in water subjected to stabilization pond treatment. The use of fecal coliforms as an indicator of the potential health hazard of the effluent may not be adequate for this treatment procedure.
我们研究了稳定塘污水处理厂中非O1群霍乱弧菌数量的时空动态。这种细菌的季节动态与粪大肠菌群相反，在炎热时期数量较高，在寒冷时期数量较低。稳定塘处理在系统进水点和出水点之间并没有显著减少非O1群霍乱弧菌的数量。相比之下，炎热时期粪大肠菌群减少了98.95%，寒冷时期减少了94.91%。在系统出水点发现，非O1群霍乱弧菌数量与温度和pH值之间的Spearman相关性的rho系数值分别为0.91和0.76，具有显著性。一项关于pH值、温度和阳光对非O1群霍乱弧菌和粪大肠菌群存活影响的实验研究证实了在稳定塘中观察到的这两种细菌群体的相反行为。pH值为8和8.8的碱性环境促进了非O1群霍乱弧菌的存活，并抑制了大肠杆菌的存活。低温（8摄氏度）延长了大肠杆菌的存活时间（k = 0.002/小时），而23摄氏度的温度则显著缩短了其存活时间（k = 0.022/小时）。在8摄氏度时，非O1群霍乱弧菌的存活情况不如大肠杆菌（k = 0.009/小时）。温度对非O1群霍乱弧菌的影响似乎与营养水平密切相关。非O1群霍乱弧菌对阳光的敏感性似乎低于大肠杆菌，尤其是在夏季，大肠杆菌的存活会显著降低。在经过稳定塘处理的水中，非O1群霍乱弧菌和大肠杆菌的行为并不相同。将粪大肠菌群用作废水潜在健康危害指标可能不适用于这种处理程序。
