Abastado J P, Lone Y C, Casrouge A, Boulot G, Kourilsky P
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Medicale U277, Paris, France.
J Exp Med. 1995 Aug 1;182(2):439-47. doi: 10.1084/jem.182.2.439.
Major histocompatibility complex (MHC) class I molecules are cell-surface proteins that present peptides to CD8+ T cells. These peptides are mostly derived from endogenously synthesized protein. Recombinant, soluble MHC class I molecules were produced, purified, and loaded homogeneously with synthetic peptide. These MHC-peptide complexes were used to activate a T cell hybridoma. While monomers of MHC-peptide bound to the T cell, they showed no stimulatory activity. Dimers fully triggered the T cell hybridoma to secrete interleukin 2. This response was followed by a state in which the T cell was refractory to restimulation as a result of defective signal transduction through the T cell receptor.
主要组织相容性复合体(MHC)I类分子是将肽段呈递给CD8+ T细胞的细胞表面蛋白。这些肽段大多来源于内源性合成的蛋白质。制备、纯化了重组可溶性MHC I类分子,并使其均匀负载合成肽。这些MHC-肽复合物用于激活T细胞杂交瘤。虽然MHC-肽单体与T细胞结合,但它们没有刺激活性。二聚体完全触发T细胞杂交瘤分泌白细胞介素2。这种反应之后会进入一种状态,由于通过T细胞受体的信号转导存在缺陷,T细胞对再次刺激变得不应答。