在胶原蛋白凝胶上培养的大鼠肝细胞合成白蛋白的过程由肝素特异性维持。

Albumin synthesis by rat hepatocytes cultured on collagen gels is sustained specifically by heparin.

作者信息

Lin K H, Hino H, Maeda S, Inagaki H, Airat J V, Saito T

机构信息

Department of Chemistry, National Industrial Research Institute of Nagoya, Japan.

出版信息

Exp Cell Res. 1995 Aug;219(2):717-21. doi: 10.1006/excr.1995.1283.

DOI:10.1006/excr.1995.1283

PMID:7641822

Abstract

We investigated the influence of various kinds of glycosaminoglycans (GAGs) in collagen gels on the maintenance of albumin synthesis in primary culture of rat hepatocytes. Among the GAGs examined (heparin, heparan sulfate, keratan sulfate, chondroitin sulfate A, dermatan sulfate, and hyaluronic acid), only heparin-containing collagen gel cultures could significantly sustain albumin synthesis. However, other GAGs, such as heparan sulfate and keratan sulfate, had almost no effect on the maintenance of albumin synthesis. Heparin in collagen gels exhibited a dose-dependent effect on albumin synthesis: heparin at 400 micrograms/ml-collagen solution maintained albumin synthesis for over 3 weeks. On the other hand, when an equivalent amount of heparin was added directly to the collagen gel culture medium, it prolonged albumin synthesis for only 10 days. The results demonstrate that specific regulation of albumin synthesis by heparin was significantly promoted by coincubating it with collagen, suggesting that some specific interaction between heparin and collagen might be of importance for the maintenance of hepatocyte functions.

摘要

我们研究了胶原凝胶中各种糖胺聚糖（GAGs）对原代培养大鼠肝细胞白蛋白合成维持的影响。在所检测的GAGs（肝素、硫酸乙酰肝素、硫酸角质素、硫酸软骨素A、硫酸皮肤素和透明质酸）中，只有含肝素的胶原凝胶培养物能显著维持白蛋白合成。然而，其他GAGs，如硫酸乙酰肝素和硫酸角质素，对白蛋白合成的维持几乎没有影响。胶原凝胶中的肝素对白蛋白合成呈现剂量依赖性效应：在400微克/毫升胶原溶液中的肝素能维持白蛋白合成超过3周。另一方面，当将等量的肝素直接添加到胶原凝胶培养基中时，它仅将白蛋白合成延长了10天。结果表明，肝素与胶原共同孵育可显著促进其对白蛋白合成的特异性调节，这表明肝素与胶原之间的某些特定相互作用可能对维持肝细胞功能很重要。