Munsterhjelm E, Khawaja J A, Eriksson O, Saris N E
Department of Medical Chemistry, University of Helsinki, Finland.
Biochem Mol Biol Int. 1995 Feb;35(2):329-36.
In this study we compare the uptake of spermine by mitochondria from Ehrlich ascites tumour cells with those from rat liver. The spermine uptake was membrane potential-dependent in both systems, but there were significant quantitative differences. The amount of spermine taken up by tumour mitochondria as a function of time was twice as large and a Lineweaver-Burk analysis showed that the Vmax was more than 6 times higher in tumour mitochondria. As in liver mitochondria, the uptake of spermine by ascites mitochondria was competitively inhibited by the polyamine spermidine and by putrescine. Mg2 was also inhibitory, as were the monovalent cations K+, Na+, Li+ and choline.
在本研究中，我们比较了艾氏腹水癌细胞线粒体与大鼠肝脏线粒体对精胺的摄取情况。在这两个系统中，精胺的摄取均依赖于膜电位，但存在显著的数量差异。肿瘤线粒体摄取精胺的量随时间变化的情况是大鼠肝脏线粒体的两倍，并且通过Lineweaver-Burk分析表明，肿瘤线粒体中的Vmax比大鼠肝脏线粒体高6倍以上。与肝脏线粒体一样，腹水线粒体对精胺的摄取受到多胺亚精胺和腐胺的竞争性抑制。Mg2+也具有抑制作用，一价阳离子K+、Na+、Li+和胆碱同样如此。
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