超氧化物反应中单线态氧的生成。
Singlet oxygen generation in the superoxide reaction.
作者信息
Mao Y, Zang L, Shi X
机构信息
Laboratory of Experimental Pathology, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
出版信息
Biochem Mol Biol Int. 1995 May;36(1):227-32.
ESR spin trapping was utilized to study the singlet oxygen (1O2) generation in the reaction of superoxide (O2) with H2O2. The spin trap used was 2,2,6,6-tetramethyl-4-piperdone. Incubation of xanthine, xanthine oxidase and H2O2 generated 1O2 spin adduct signal. Omission of xanthine, xanthine oxidase or H2O2 caused a sharp decrease in 1O2 generation. 1O2 scavenger, sodium azide, inhibited 1O2 generation while .OH scavenger, ethanol, only slightly decreased the signal intensity. Potassium superoxide (KO2) decomposition generated 1O2. Catalase and sodium azide inhibited 1O2 generation and H2O2 enhanced it. The results demonstrate that O2 is capable of generating 1O2 upon reaction with H2O2.
电子自旋捕获技术被用于研究超氧化物（O₂）与过氧化氢（H₂O₂）反应过程中单线态氧（¹O₂）的生成。所使用的自旋捕获剂是2,2,6,6 - 四甲基 - 4 - 哌啶酮。黄嘌呤、黄嘌呤氧化酶与H₂O₂共同孵育产生了¹O₂自旋加合物信号。省略黄嘌呤、黄嘌呤氧化酶或H₂O₂会导致¹O₂生成量急剧下降。¹O₂清除剂叠氮化钠抑制了¹O₂的生成，而·OH清除剂乙醇仅略微降低了信号强度。超氧化钾（KO₂）分解产生了¹O₂。过氧化氢酶和叠氮化钠抑制了¹O₂的生成，而H₂O₂则增强了其生成。结果表明，O₂与H₂O₂反应时能够生成¹O₂。
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