Leclerc F, Cedergren R, Ellington A D
Département de biochimie, Université de Montréal, Québec, Canada.
Nat Struct Biol. 1994 May;1(5):293-300. doi: 10.1038/nsb0594-293.
Coordinated variations in the sequence of the Rev-binding element of HIV-1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three-dimensional models of the wild-type Rev-binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non-Watson-Crick pairings open the major groove allowing access of an alpha-helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.
通过体外基因筛选鉴定出的HIV-1 Rev结合元件序列中的协同变异，已被用作分子建模的距离和构象限制。构建了野生型Rev结合元件和几种进化后的RNA配体（适体）的三维模型。这些模型表明，非沃森-克里克配对打开了大沟，允许Rev的α-螺旋肽进入，并解释了为什么一些选定的RNA序列能比其他序列更紧密地结合Rev。
