Blanchet-Tournier M F, Tricoire H, Busson D, Lamour-Isnard C
Institut Jacques Monod, Université Paris VII, France.
Gene. 1995 Aug 19;161(2):157-62. doi: 10.1016/0378-1119(95)00219-v.
The segment polarity gene fused (fu) is involved in specification of positional information inside embryonic segments in Drosophila melanogaster (Dm). The predicted Fused (Fu) protein contains a serine/threonine kinase domain and a second domain with unknown function. We cloned and sequenced the fu homologous gene from Drosophila virilis (Dv) and made an interspecific DNA sequence comparison to identify regions that have been conserved during evolution. Comparison of the predicted amino acid (aa) sequences reveals two regions of strong homology, one corresponding to the kinase domain (268 aa), the other located in the third exon of the Dm fu gene, suggesting a functional importance for this region. Stretches of significantly conserved sequences are also observed in the 5' and 3' untranslated regions. Weak homology is seen in the intronic sequences although the adjacent exonic sequences are mostly conserved. These findings indicate a high conservation of the predicted Fu protein during the evolution of Drosophila.
节段极性基因融合（fu）参与黑腹果蝇（Dm）胚胎节段内位置信息的特化。预测的融合（Fu）蛋白包含一个丝氨酸/苏氨酸激酶结构域和一个功能未知的第二个结构域。我们从粗壮果蝇（Dv）中克隆并测序了fu同源基因，并进行了种间DNA序列比较，以确定在进化过程中保守的区域。预测的氨基酸（aa）序列比较揭示了两个高度同源的区域，一个对应于激酶结构域（268个氨基酸），另一个位于Dm fu基因的第三个外显子中，表明该区域具有功能重要性。在5'和3'非翻译区也观察到显著保守序列的片段。尽管相邻的外显子序列大多保守，但内含子序列中可见弱同源性。这些发现表明在果蝇进化过程中预测的Fu蛋白具有高度保守性。
