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在剑尾鱼 - 新月鱼系统(新月鱼 - 剑尾鱼)中实现基因转化的可能性。

A possibility to achieve genetic transformation in the platyfish-swordtail system (Platypoecilus maculatus - Xiphophorus helleri).

作者信息

Schwab M, Vielkind J, Anders F

出版信息

Experientia. 1976 Mar 15;32(3):301-2. doi: 10.1007/BF01940803.

DOI:10.1007/BF01940803
PMID:767130
Abstract

In order to determine how informative homologous donor DNA might be made available to propigment cells of the recipient Xiphophorus helleri for transformation, labelled heterologous DNA from E. coli was injected into the neural crest region or the yolk sac of embryos of the recipient. On the basis of the degradation rate of the donor DNA and the incorporation rate of radioactivity into the recipient DNA, it is concluded that injection into the neural crest region may be a suitable method to make available informative homologous donor DNA for transformation.

摘要

为了确定如何使同源供体DNA能够为受体剑尾鱼(Xiphophorus helleri)的色素生成细胞提供用于转化的信息,将来自大肠杆菌的标记异源DNA注射到受体胚胎的神经嵴区域或卵黄囊中。根据供体DNA的降解率以及放射性掺入受体DNA的速率,得出结论:注射到神经嵴区域可能是一种为转化提供信息丰富的同源供体DNA的合适方法。

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1
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本文引用的文献

1
STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES : INDUCTION OF TRANSFORMATION BY A DESOXYRIBONUCLEIC ACID FRACTION ISOLATED FROM PNEUMOCOCCUS TYPE III.肺炎球菌型转变物质的化学性质研究:从 III 型肺炎球菌中分离出的脱氧核糖核酸片段诱导转化。
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2
Tumour formation in platyfish-swordtail hybrids as a problem of gene regulation.剑尾鱼 - 新月鱼杂交种中的肿瘤形成作为一个基因调控问题
Experientia. 1967 Jan 15;23(1):1-10. doi: 10.1007/BF02142235.
3
Uptake of DNA by living cells.
活细胞对DNA的摄取。
Prog Nucleic Acid Res Mol Biol. 1965;4:231-67. doi: 10.1016/s0079-6603(08)60790-4.
4
Uptake of bacterial H3-DNA into fish embryos.
Experientia. 1971 Mar 15;27(3):347-8. doi: 10.1007/BF02138190.
5
Transformationlike phenomena in somatic cells.
Int Rev Cytol. 1968;23:1-23. doi: 10.1016/s0074-7696(08)60268-5.