Burris T P, Guo W, Le T, McCabe E R
Department of Pediatrics, UCLA School of Medicine 90024, USA.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):576-81. doi: 10.1006/bbrc.1995.2324.
The nuclear hormone receptor, DAX-1, is responsible for X-linked adrenal hypoplasia congenita and hypogonadotrophic hypogonadism. We recently cloned the 5' flanking region of the human DAX-1 gene and in this report we describe the identification of a putative steroidogenic factor 1 (SF-1) response element approximately 110 bases upstream of the TATA box. Both DAX-1 and SF-1 are expressed in similar tissues including the adrenal cortex, gonads, hypothalamus, and the pituitary gland. Like DAX-1, SF-1 expression has been shown to be essential for the development of the adrenal cortex. We demonstrate that SF-1 is able to efficiently bind to the putative SF-1 response element found in the DAX-1 promoter in vitro. This suggests that SF-1 may directly regulate the expression of DAX-1 and that these two transcription factors may be components of a cascade required for development of steroidogenic tissues.
核激素受体DAX-1与X连锁先天性肾上腺发育不全及低促性腺激素性性腺功能减退有关。我们最近克隆了人类DAX-1基因的5'侧翼区，在本报告中，我们描述了在TATA框上游约110个碱基处鉴定出一个假定的类固醇生成因子1（SF-1）反应元件。DAX-1和SF-1均在包括肾上腺皮质、性腺、下丘脑和垂体在内的相似组织中表达。与DAX-1一样，SF-1的表达已被证明对肾上腺皮质的发育至关重要。我们证明，在体外，SF-1能够有效结合DAX-1启动子中发现的假定SF-1反应元件。这表明SF-1可能直接调节DAX-1的表达，并且这两种转录因子可能是类固醇生成组织发育所需级联反应的组成部分。
