采用气相色谱-质谱联用技术鉴定尿液水解产物中的O-磷酸氨基酸。

Identification of O-phosphoamino acids in urine hydrolysate by gas chromatography-mass spectrometry.

作者信息

Kataoka H, Nakai K, Makita M

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

J Chromatogr. 1993 May 19;615(1):136-41. doi: 10.1016/0378-4347(93)80299-j.

DOI:10.1016/0378-4347(93)80299-j

PMID:7688003

Abstract

The occurrence of O-phosphoserine (P-Ser), O-phosphothreonine (P-Thr) and O-phosphotyrosine (P-Tyr) in the hydrolysate of human urine was demonstrated. Urine samples were separated into non-adsorbed and adsorbed fractions by DEAE-cellulose column chromatography. After acid and base hydrolyses of these fractions, the O-phosphoamino acids in the hydrolysates were converted into their N-isobutoxycarbonyl methyl ester derivatives and identified by gas chromatography-mass spectrometry. By gas chromatography with flame photometric detection, the urinary contents of P-Ser, P-Thr and P-Tyr were estimated to be 945 +/- 3, 109 +/- 3 and 2.9 +/- 0.2 ng/ml, respectively.

摘要

已证实人尿水解产物中存在O-磷酸丝氨酸（P-Ser）、O-磷酸苏氨酸（P-Thr）和O-磷酸酪氨酸（P-Tyr）。通过DEAE-纤维素柱色谱法将尿样分离为未吸附和吸附部分。对这些部分进行酸碱水解后，将水解产物中的O-磷酸氨基酸转化为其N-异丁氧羰基甲酯衍生物，并通过气相色谱-质谱法进行鉴定。通过带有火焰光度检测的气相色谱法，估计尿中P-Ser、P-Thr和P-Tyr的含量分别为945±3、109±3和2.9±0.2 ng/ml。