精氨酸酶在青蛙肝脏和肾脏提取物中的凝胶过滤行为。
Gel filtration behaviour of arginase in frog liver and kidney extracts.
作者信息
Venkatakrishnan G, Reddy S R
机构信息
Department of Zoology, University of Poona, Pune Maharastra, India.
出版信息
Arch Int Physiol Biochim Biophys. 1993 Mar-Apr;101(2):141-3. doi: 10.3109/13813459309008883.
The molecular weight of arginase, determined by gel filtration of frog (Rana tigerina) liver and kidney extracts, varied with the protein concentration. It increased from about 25-27 KDa in dilute solutions to about 92-96 KDA in concentrated extracts. In addition, an arginase with a molecular weight of 45-49 KDA appeared at intermediate concentrations. The results suggest the probable aggregation of arginase monomers to yield the dimeric and tetrameric forms of the enzyme at high protein concentration in extracts.
通过对虎纹蛙肝脏和肾脏提取物进行凝胶过滤测定，精氨酸酶的分子量随蛋白质浓度而变化。在稀溶液中，其分子量约为25 - 27千道尔顿，在浓缩提取物中则增至约92 - 96千道尔顿。此外，在中等浓度下出现了分子量为45 - 49千道尔顿的精氨酸酶。结果表明，在提取物中高蛋白浓度下，精氨酸酶单体可能会聚集形成该酶的二聚体和四聚体形式。
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