Fazleabas A T, Donnelly K M, Mavrogianis P A, Verhage H G
Department of Obstetrics and Gynecology, University of Illinois, College of Medicine at Chicago 60680.
Biol Reprod. 1993 Oct;49(4):695-704. doi: 10.1095/biolreprod49.4.695.
The study was undertaken to characterize changes in uterine endometrial secretory activity and morphology in the baboon between Days 18 and 32 of pregnancy. Proteins synthesized by the implantation site endometrium (RI) and non-implantation site endometrium (RIII) and developing placenta were analyzed by two-dimensional PAGE and fluorography. The electrophoretic mobility and localization of chorionic gonadotropin (CG) in the baboon placenta, and insulin-like growth factor binding protein-1 (IGFBP-1) and retinol binding protein (RBP) in the baboon uterus were confirmed by Western blot analysis and immunocytochemistry. Placental protein synthesis was primarily characterized by the presence of radiolabeled CG at Days 18 and 25, which decreased by Day 32. Peak CG synthetic activity, immunolocalized to the syncytiotrophoblast, occurred during the formation of tertiary villi. Also associated with the development of mature villi was a corresponding increase in a basic protein (M(r) 23,000). The overall secretory pattern of the baboon endometrium was similar to that observed during the midluteal stage; however, the synthetic activity was enhanced during pregnancy. Radiolabeled and immunoreactive IGFBP-1 and RBP were detectable in the Day 32 functionalis and basalis, respectively, and their synthesis was higher in RI compared to RIII. The immunolocalization of IGFBP-1 in RI appeared to be directly related to the degree of decidualization. In addition, a novel group of low molecular-weight (M(r) 27,000-30,000) isoelectrovariant proteins was secreted predominantly by the basalis at Days 18 and 25 of pregnancy and disappeared by Day 32 in all but one animal. The synthesis of this group of proteins did not show regional specificity. These studies indicate that the baboon endometrium undergoes morphological and secretory changes during pregnancy that are associated with the development of a functional placenta and decidualized endometrium. Furthermore, these studies suggest that the conceptus/placenta may mediate specific changes in IGFBP-1 and RBP expression at the implantation site.
本研究旨在描述狒狒妊娠第18天至32天期间子宫内膜分泌活动和形态的变化。通过二维聚丙烯酰胺凝胶电泳和荧光自显影分析着床部位子宫内膜（RI）、非着床部位子宫内膜（RIII）以及发育中的胎盘所合成的蛋白质。通过蛋白质免疫印迹分析和免疫细胞化学方法，证实了狒狒胎盘绒毛膜促性腺激素（CG）的电泳迁移率和定位，以及狒狒子宫中胰岛素样生长因子结合蛋白-1（IGFBP-1）和视黄醇结合蛋白（RBP）的情况。胎盘蛋白合成的主要特征是在第18天和第25天存在放射性标记的CG，到第32天时减少。CG合成活性峰值定位于合体滋养层，出现在三级绒毛形成期间。与成熟绒毛发育相关的还有一种碱性蛋白（相对分子质量23,000）相应增加。狒狒子宫内膜的总体分泌模式与黄体中期观察到的相似；然而，妊娠期间合成活性增强。在第32天的功能层和基底层分别可检测到放射性标记和免疫反应性的IGFBP-1和RBP，且RI中它们的合成高于RIII。IGFBP-1在RI中的免疫定位似乎与蜕膜化程度直接相关。此外，一组新的低分子量（相对分子质量27,000 - 30,000）等电变体蛋白在妊娠第18天和第25天主要由基底层分泌，除一只动物外，到第32天消失。这组蛋白的合成没有显示出区域特异性。这些研究表明，狒狒子宫内膜在妊娠期间会发生形态和分泌变化，这些变化与功能性胎盘和蜕膜化子宫内膜的发育相关。此外，这些研究表明，孕体/胎盘可能介导着床部位IGFBP-1和RBP表达的特定变化。
