可变聚腺苷酸化产生三种低等电点的α-淀粉酶mRNA，它们在大麦中具有差异表达。
Alternative polyadenylation generates three low-pI alpha-amylase mRNAs with differential expression in barley.
作者信息
Skadsen R W, Knauer N S
机构信息
United States Department of Agriculture, Agricultural Research Service, Madison, WI 53705.
出版信息
FEBS Lett. 1995 Mar 20;361(2-3):220-4. doi: 10.1016/0014-5793(95)00112-m.
Specific low-pI alpha-amylase genes from barley (Hordeum vulgare L.) produced alternative mRNAs with a 17-base 3' extension (extension 1) or a 17-base extension beyond this (extension 2). The extended mRNAs do not arise from splicing of downstream sequences, and not all low-pI genes contain the extended sequences. All three mRNAs occur in aleurones and shoots, while extension 2 is missing from scutella. Also, the unextended mRNAs predominate in total mRNA, but the extended mRNAs predominate in membrane-bound polysomes. The extended sequences do not occur in previously characterized alpha-amylases, but 16 of 18 bases, mainly in extension 1, are identical with a sequence in the 3'-UTR of PAPI, a putative inhibitor of alpha-amylase. These observations suggest that the extended sequences could play a functional role in alpha-amylase expression.
来自大麦（Hordeum vulgare L.）的特定低等电点α-淀粉酶基因产生了具有17个碱基3'端延伸（延伸1）或在此基础上再延伸17个碱基（延伸2）的可变mRNA。延伸的mRNA并非来自下游序列的剪接，而且并非所有低等电点基因都含有延伸序列。这三种mRNA均存在于糊粉层和芽中，而盾片中缺少延伸2。此外，未延伸的mRNA在总mRNA中占主导，但延伸的mRNA在膜结合多核糖体中占主导。延伸序列在先前已鉴定的α-淀粉酶中不存在，但18个碱基中的16个，主要在延伸1中，与一种假定的α-淀粉酶抑制剂PAPI的3'-UTR中的一个序列相同。这些观察结果表明，延伸序列可能在α-淀粉酶表达中发挥功能性作用。
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