Fiddler M, Abdel-Rahman B, Rappolee D A, Pergament E
Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, Illinois, USA.
Am J Med Genet. 1995 Jan 2;55(1):80-4. doi: 10.1002/ajmg.1320550121.
We have examined the expression of SRY mRNA in individual in vitro fertilized preimplantation human embryos; because of ethical constraints, these studies were confined to embryos with one and three pronuclei. Using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we observed SRY mRNA at the one-cell through the blastula stages but not in spermatozoa. These results indicate that the de novo transcription of this sex-specific gene occurs at a developmental time considerably earlier than that of gonadal differentiation. Our results also indicate that in vitro fertilized embryos with one pronucleus are likely to be diploid.
我们检测了体外受精的植入前人类胚胎个体中SRY mRNA的表达；由于伦理限制，这些研究仅限于具有一个和三个原核的胚胎。使用灵敏的逆转录聚合酶链反应（RT-PCR）检测方法，我们在单细胞期至囊胚期的胚胎中观察到了SRY mRNA，但在精子中未观察到。这些结果表明，这个性别特异性基因的从头转录发生的发育时间比性腺分化的时间要早得多。我们的结果还表明，具有一个原核的体外受精胚胎很可能是二倍体。
