Bernstein J J, Woodard C A
Laboratory of Central Nervous System Regeneration and Neuro-Oncology, Department of Veterans Affairs Medical Center, Washington, District of Columbia.
Neurosurgery. 1995 Jan;36(1):124-32; discussion 132. doi: 10.1227/00006123-199501000-00016.
Glioblastoma is very rarely found outside the central nervous system. The ability of rat C6 glioblastoma cells to intravasate into central nervous system and pial blood vessels is tested using a rat homografting model and two in vitro models. In vivo, scanning electron microscopy demonstrates that upon grafting C6 cells into implantation pockets in rat cortex, blood vessels can be spared in large digestion cysts formed in host brain parenchyma. Immunocytochemistry of the grafted rat cortex reveals that the glioblastoma cells are upon the blood vessel basement membrane, surrounded by the extracellular matrix material, fibronectin. The endothelial cells of the blood vessel are inside the laminin and fibronectin, and there were areas of endothelial cell hyperplasia. C6 cells are not observed inside blood vessels. In vitro, C6 cell cultures seeded with blood vessels from fresh rat pia exhibit the same relationship of the C6 glioblastoma cells to the blood vessel as those in the other models. The C6 cells migrate upon the pial blood vessel basement membrane but do not intravasate into the blood vessel. To ascertain whether structure and components of the blood vessel basement membrane are important factors in glioblastoma cell exclusion from blood vessels, C6 cells are seeded upon artificial basement membrane hydrated gel wafers. C6 cells migrate into the artificial basement membrane gel wafer by 1 day after seeding. These data indicate that glioblastoma cells are confined to the central nervous system by an inability to pass through vital basement membrane.
胶质母细胞瘤在中枢神经系统之外极为罕见。利用大鼠同种移植模型和两种体外模型，对大鼠C6胶质母细胞瘤细胞侵入中枢神经系统和软脑膜血管的能力进行了测试。在体内，扫描电子显微镜显示，将C6细胞移植到大鼠皮质的植入囊中后，宿主脑实质中形成的大消化囊肿中的血管可以幸免。移植大鼠皮质的免疫细胞化学显示，胶质母细胞瘤细胞位于血管基底膜上，被细胞外基质物质纤连蛋白包围。血管内皮细胞位于层粘连蛋白和纤连蛋白内部，存在内皮细胞增生区域。未在血管内观察到C6细胞。在体外，接种新鲜大鼠软脑膜血管的C6细胞培养物中，C6胶质母细胞瘤细胞与血管的关系与其他模型相同。C6细胞在软脑膜血管基底膜上迁移，但不侵入血管。为了确定血管基底膜的结构和成分是否是胶质母细胞瘤细胞被血管排斥的重要因素，将C6细胞接种在人工基底膜水合凝胶薄片上。接种后1天，C6细胞迁移到人工基底膜凝胶薄片中。这些数据表明，胶质母细胞瘤细胞因无法穿过重要的基底膜而局限于中枢神经系统。
