Sykes P A, Boulter K H, Schofield P F
J Med Microbiol. 1976 Feb;9(1):13-22. doi: 10.1099/00222615-9-1-13.
Small-bowel content was examined bacteriologically whilst fresh and after storage for 24, 48, and 72 h in a modified Stuart's medium. There was little alteration in the viable count of individual intestinal organisms. For accurate quantitative analysis, 0-3 ml of intestinal content was enough. Specimens of small-bowel content were obtained by needle aspiration, and a qualitative and quantitative study was made of the microflora of patients with acute intestinal obstruction and of a control group of patients. Results in the control group confirmed the findings of the results of intubation studies by other workers, that a quantitative gradient of aerobic and anaerobic organisms exists from jejunum to distal ileum. In acute small-bowel obstruction and acute large-bowel obstruction there was a loss of the normal gradient and an increase in the absolute numbers of organisms present; this was particularly marked for anaerobic organisms in large-bowel obstruction.
对小肠内容物进行了细菌学检查，先是新鲜时检查，然后将其在改良的斯图尔特培养基中储存24、48和72小时后再检查。单个肠道微生物的活菌数几乎没有变化。为进行准确的定量分析，0 - 3毫升的肠道内容物就足够了。通过针吸获取小肠内容物标本，并对急性肠梗阻患者和对照组患者的微生物群进行了定性和定量研究。对照组的结果证实了其他研究人员插管研究的结果，即从空肠到回肠末端存在需氧菌和厌氧菌的定量梯度。在急性小肠梗阻和急性大肠梗阻中，正常梯度消失，存在的微生物绝对数量增加；这在大肠梗阻的厌氧菌中尤为明显。
