Machold J, Weise C, Utkin Y N, Franke P, Tsetlin V I, Hucho F
Institut für Biochemie, Freie Universität Berlin, Germany.
Eur J Biochem. 1995 Mar 15;228(3):947-54. doi: 10.1111/j.1432-1033.1995.tb20345.x.
A new series of photoactivatable and cleavable derivatives of neurotoxin II from the cobra Naja naja oxiana is investigated which can be used for mapping the surface topology of the nicotinic acetylcholine receptor from Torpedo electric tissue. The preparation and characterisation of five toxin derivatives, each with a radioactive 125I-azidosalicylamidoethyl-1,3'-dithiopropyl group in a defined position within the primary structure, are described. The photoinduced cross-linking reaction of the toxin derivatives with membrane-bound receptor is investigated. The photoactivatable group located at position K25 reacts almost exclusively with the delta subunit of the receptor, whereas the K15 derivative reacts with the alpha and beta subunits. The other derivatives did not react with the receptor to any significant extent. It is shown that, with respect to the receptor subunits, the cross-linking pattern depends on the length and chemical nature of the cross-linking group.
研究了一系列来自中亚眼镜蛇神经毒素II的新型光可激活和可裂解衍生物，这些衍生物可用于绘制电鳐电组织中烟碱型乙酰胆碱受体的表面拓扑结构。描述了五种毒素衍生物的制备和表征，每种衍生物在一级结构的特定位置都带有放射性125I-叠氮水杨酰胺基乙基-1,3'-二硫代丙基基团。研究了毒素衍生物与膜结合受体的光诱导交联反应。位于K25位置的光可激活基团几乎只与受体的δ亚基反应，而K15衍生物与α和β亚基反应。其他衍生物与受体没有明显反应。结果表明，就受体亚基而言，交联模式取决于交联基团的长度和化学性质。
