Myoga H, Nonaka S, Matsuyama K, Mori S
Department of Otolaryngology, Asahikawa Medical College, Japan.
Neurosci Res. 1995 Jan;21(3):211-21. doi: 10.1016/0168-0102(94)00857-c.
The contribution of the serotonergic system to postnatal development of locomotor movements in newborn Sprague-Dawley rats was studied from PND 1 to PND 15. To deplete serotonin (5-HT), p-chlorophenylalanine (PCPA) (200 mg/kg) was intraperitoneally administered at PND 1, 4, 7, 10 and 13, and the results obtained from PCPA-treated pups (n = 20) and control pups (n = 20) were compared. Locomotor movements of the pups on the surface of a digitizer table were measured by attaching a miniature coil to the abdomen of the pups. Video-recordings were made to study the locomotor movements. From PND 1 to PND 5, no significant difference in body weights and locomotor movements was observed across control and PCPA-treated pups. After PND 6, the development of PCPA-treated pups was slightly retarded although both groups of pups opened their eyes at PND 14. Throughout PND 6 to PND 15, the total distance and mean velocity of locomotor movements were significantly lower in PCPA-treated pups than in the control pups. In addition, forelimb and hindlimb movements were not well coordinated in the former. These results suggest that retardation of locomotor movements in the PCPA-treated pups is due to a possible failure in the postnatal development of 5-HT and other neural mechanisms related to postural and locomotor control, and not due to that of general development of the pups themselves.
从出生后第1天（PND 1）到第15天，研究了血清素能系统对新生斯普拉格-道利大鼠运动发育的贡献。为了耗尽血清素（5-HT），在出生后第1、4、7、10和13天腹腔注射对氯苯丙氨酸（PCPA）（200mg/kg），并比较了PCPA处理的幼崽（n = 20）和对照幼崽（n = 20）的结果。通过将一个微型线圈附着在幼崽的腹部来测量数字化仪台上幼崽的运动。进行视频记录以研究运动。从出生后第1天到第5天，对照幼崽和PCPA处理的幼崽在体重和运动方面没有观察到显著差异。出生后第6天之后，PCPA处理的幼崽发育略有迟缓，尽管两组幼崽均在出生后第14天睁眼。在整个出生后第6天到第15天期间，PCPA处理的幼崽的运动总距离和平均速度显著低于对照幼崽。此外，前者的前肢和后肢运动协调性不佳。这些结果表明，PCPA处理的幼崽运动迟缓是由于5-HT出生后发育以及与姿势和运动控制相关的其他神经机制可能出现故障，而不是由于幼崽自身的一般发育出现故障。
