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Protein and lipid rotational dynamics in cardiac and skeletal sarcoplasmic reticulum detected by EPR and phosphorescence anisotropy.
Biochemistry. 1993 Sep 14;32(36):9445-53. doi: 10.1021/bi00087a024.
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Molecular mechanism of Ca-ATPase activation by halothane in sarcoplasmic reticulum.氟烷激活肌浆网中钙-ATP酶的分子机制。
Biochemistry. 1993 Jul 27;32(29):7503-11. doi: 10.1021/bi00080a023.
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pH dependence of kinetics and steady-state block of cardiac sodium channels by lidocaine.
Am J Physiol. 1993 May;264(5 Pt 2):H1588-98. doi: 10.1152/ajpheart.1993.264.5.H1588.
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Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+.
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Depth profiling of dibucaine in sarcoplasmic reticulum vesicles by fluorescence quenching.通过荧光猝灭对肌浆网囊泡中丁卡因进行深度剖析。
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Biochemistry. 1994 May 3;33(17):5056-62. doi: 10.1021/bi00183a008.
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Mechanism of inhibition of the Ca(2+)-ATPase by spermine and other polycationic compounds.精胺及其他聚阳离子化合物对Ca(2+)-ATP酶的抑制机制。
Biochemistry. 1994 Apr 26;33(16):4745-54. doi: 10.1021/bi00182a001.
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Effects of membrane thickness on the molecular dynamics and enzymatic activity of reconstituted Ca-ATPase.膜厚度对重组钙-ATP酶分子动力学和酶活性的影响。
Biochemistry. 1994 Mar 15;33(10):2912-20. doi: 10.1021/bi00176a022.
9
Halothane and cyclopiazonic acid modulate Ca-ATPase oligomeric state and function in sarcoplasmic reticulum.氟烷和环匹阿尼酸调节肌浆网中钙-ATP酶的寡聚状态和功能。
Biochemistry. 1994 Nov 22;33(46):13928-37. doi: 10.1021/bi00250a048.
10
Hexanol and lidocaine affect the oligomeric state of the Ca-ATPase of sarcoplasmic reticulum.己醇和利多卡因影响肌浆网Ca-ATP酶的寡聚状态。
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麻醉剂会改变心肌肌浆网中钙-ATP酶的物理和功能特性。

Anesthetics alter the physical and functional properties of the Ca-ATPase in cardiac sarcoplasmic reticulum.

作者信息

Karon B S, Geddis L M, Kutchai H, Thomas D D

机构信息

Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455, USA.

出版信息

Biophys J. 1995 Mar;68(3):936-45. doi: 10.1016/S0006-3495(95)80269-9.

DOI:10.1016/S0006-3495(95)80269-9
PMID:7756557
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1281817/
Abstract

We have studied the effects of the local anesthetic lidocaine, and the general anesthetic halothane, on the function and oligomeric state of the CA-ATPase in cardiac sarcoplasmic reticulum (SR). Oligomeric changes were detected by time-resolved phosphorescence anisotropy (TPA). Lidocaine inhibited and aggregated the Ca-ATPase in cardiac SR. Micromolar calcium or 0.5 M lithium chloride protected against lidocaine-induced inhibition, indicating that electrostatic interactions are essential to lidocaine inhibition of the Ca-ATPase. The phospholamban (PLB) antibody 2D12, which mimics PLB phosphorylation, had no effect on lidocaine inhibition of the Ca-ATPase in cardiac SR. Inhibition and aggregation of the Ca-ATPase in cardiac SR occurred at lower concentrations of lidocaine than necessary to inhibit and aggregate the Ca-ATPase in skeletal SR, suggesting that the cardiac isoform of the enzyme has a higher affinity for lidocaine. Halothane inhibited and aggregated the Ca-ATPase in cardiac SR. Both inhibition and aggregation of the Ca-ATPase by halothane were much greater in the presence of PLB antibody or when PLB was phosphorylated, indicating a protective effect of PLB on halothane-induced inhibition and aggregation. The effects of halothane on cardiac SR are opposite from the effects of halothane observed in skeletal SR, where halothane activates and dissociates the Ca-ATPase. These results underscore the crucial role of protein-protein interactions on Ca-ATPase regulation and anesthetic perturbation of cardiac SR.

摘要

我们研究了局部麻醉药利多卡因和全身麻醉药氟烷对心肌肌浆网（SR）中钙-ATP酶功能和寡聚状态的影响。通过时间分辨磷光 anisotropy（TPA）检测寡聚变化。利多卡因抑制并聚集心肌SR中的钙-ATP酶。微摩尔浓度的钙或0.5 M氯化锂可防止利多卡因诱导的抑制作用，表明静电相互作用对利多卡因抑制钙-ATP酶至关重要。模拟磷蛋白磷酸化的磷蛋白（PLB）抗体2D12对利多卡因抑制心肌SR中的钙-ATP酶没有影响。心肌SR中钙-ATP酶的抑制和聚集在低于抑制和聚集骨骼肌SR中钙-ATP酶所需浓度的利多卡因下发生，这表明该酶的心脏同工型对利多卡因具有更高的亲和力。氟烷抑制并聚集心肌SR中的钙-ATP酶。在存在PLB抗体或PLB磷酸化时，氟烷对钙-ATP酶的抑制和聚集作用都大得多，表明PLB对氟烷诱导的抑制和聚集具有保护作用。氟烷对心肌SR的影响与在骨骼肌SR中观察到的氟烷的影响相反，在骨骼肌SR中氟烷激活并解离钙-ATP酶。这些结果强调了蛋白质-蛋白质相互作用在钙-ATP酶调节和心肌SR麻醉扰动中的关键作用。