亲硫吸附在生物素化Fab片段纯化中的应用。

Use of thiophilic adsorption in the purification of biotinylated Fab fragments.

作者信息

Lutomski D, Joubert-Caron R, Bourin P, Bladier D, Caron M

机构信息

Laboratoire de Biochimie et Technologie des Protéines, UFR Léonard de Vinci, Université Paris Nord, Bobigny, France.

出版信息

J Chromatogr B Biomed Appl. 1995 Feb 3;664(1):79-82. doi: 10.1016/0378-4347(94)00399-p.

DOI:10.1016/0378-4347(94)00399-p

PMID:7757243

Abstract

A method for the purification and biotinylation of Fab fragments, using thiophilic adsorption (T-gel), is described. The T-gel was used to purify an IgG fraction directly in the buffer suitable for biotinylation, and to adsorb intact IgGs and papain after enzymatic digestion. For the final step, Fc fragments were removed with a protein A column.

摘要

描述了一种使用嗜硫吸附（T-凝胶）对Fab片段进行纯化和生物素化的方法。T-凝胶用于在适合生物素化的缓冲液中直接纯化IgG组分，并在酶消化后吸附完整的IgG和木瓜蛋白酶。在最后一步中，用蛋白A柱去除Fc片段。