复苏条件对蜡样芽孢杆菌孢子表观耐热性的影响。
The effect of recovery conditions on the apparent heat resistance of Bacillus cereus spores.
作者信息
Gonzalez I, Lopez M, Mazas M, Gonzalez J, Bernardo A
机构信息
Tecnología de los Alimentos, Facultad de Veterinaria, León, Spain.
出版信息
J Appl Bacteriol. 1995 May;78(5):548-54. doi: 10.1111/j.1365-2672.1995.tb03098.x.
The effect of recovery media and incubation temperature on the apparent heat resistance of three ATCC strains (4342, 7004 and 9818) of Bacillus cereus spores were studied. Nutrient Agar (NA), Tryptic Soy Agar (TSA), Plate Count Agar (PCA) and Milk Agar (MA) as the media and temperatures in the range of 15-40 degrees C were used to recover heated spores. Higher counts of heat injured spores were obtained on PCA and NA. The optimum subculture temperature was about 5 degrees C below the optimum temperature for unheated spores. No significant differences in heat resistance were observed with the different recovery conditions except for strains 4342 and 9818 when MA was used as plating medium. Large differences in D-values were found among the strains (D100 = 0.28 min for 7004; D100 = 0.99 min for 4342; D100 = 4.57 min for 9818). The 7004 strain showed a sub-population with a greater heat resistance. The z values obtained for the three strains studied under the different recovery conditions were similar (7.64 degrees C +/- 0.25).
研究了复苏培养基和培养温度对蜡样芽孢杆菌3株ATCC菌株（4342、7004和9818）芽孢表观耐热性的影响。使用营养琼脂（NA）、胰蛋白胨大豆琼脂（TSA）、平板计数琼脂（PCA）和乳琼脂（MA）作为培养基，并在15 - 40摄氏度范围内的温度下复苏加热后的芽孢。在PCA和NA上获得的热损伤芽孢数量更多。最佳传代培养温度比未加热芽孢的最佳温度低约5摄氏度。当使用MA作为平板培养基时，除了菌株4342和9818外，不同复苏条件下未观察到耐热性的显著差异。各菌株之间的D值存在很大差异（7004菌株的D100 = 0.28分钟；4342菌株的D100 = 0.99分钟；9818菌株的D100 = 4.57分钟）。7004菌株显示出一个具有更高耐热性的亚群。在不同复苏条件下研究的这3株菌株获得的z值相似（7.64摄氏度±0.25）。
Zotero 插件