通过一级结构鉴定出的俄罗斯鲟（Acipenser gueldenstaedti）中的哺乳动物促性腺激素释放激素（GnRH）。

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.

作者信息

Lescheid D W, Powell J F, Fischer W H, Park M, Craig A, Bukovskaya O, Barannikova I A, Sherwood N M

机构信息

Department of Biology, University of Victoria, B.C., Canada.

出版信息

Regul Pept. 1995 Feb 14;55(3):299-309. doi: 10.1016/0167-0115(94)00118-h.

DOI:10.1016/0167-0115(94)00118-h

PMID:7761629

Abstract

The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

摘要

利用反相高效液相色谱法（HPLC）从俄罗斯鲟鱼（Acipenser gueldenstaedti）的大脑中纯化出哺乳动物形式的促性腺激素释放激素（GnRH）。这些鱼体内mGnRH的总浓度为5.4纳克/脑。还检测到少量免疫反应性鸡GnRH-II样分子，但数量不足以进行纯化。mGnRH的一级结构通过自动Edman降解法确定。由于在牛焦谷氨酸氨基肽酶消化后才能获得序列数据，因此确定氨基末端残基被修饰。此外，质谱数据以及在HPLC上与合成mGnRH的共洗脱证实羧基末端残基被酰胺化。鲟鱼GnRH的氨基酸序列为pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2。