互补DNA测序：一种理解细胞生理学的方法。
cDNA sequencing: a means of understanding cellular physiology.
作者信息
Weinstock K G, Kirkness E F, Lee N H, Earle-Hughes J A, Venter J C
机构信息
Institute for Genomic Research, Gaithersburg.
出版信息
Curr Opin Biotechnol. 1994 Dec;5(6):599-603. doi: 10.1016/0958-1669(94)90081-7.
PMID:7765742
Abstract
High-throughput automated sequencing has enabled researchers to examine large numbers of clones from a cDNA library as a measure of the steady-state levels of mRNA species. The past year has witnessed many new applications of this technique to allow the qualitative and quantitative comparison of the changes in transcript levels from multiple genes.
摘要
高通量自动化测序使研究人员能够检测来自cDNA文库的大量克隆，以此作为衡量mRNA种类稳态水平的一种方法。在过去的一年里，这项技术有了许多新的应用，可用于对多个基因转录水平变化进行定性和定量比较。
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