Zakova N, Szatmari G B
Département de microbiologie et immunologie, Université de Montréal, Québec, Canada.
Mol Gen Genet. 1995 May 20;247(4):509-14. doi: 10.1007/BF00293154.
The site-specific recombination system used by multicopy plasmids of the ColE1 family uses two identical plasmid-encoded recombination sites and four bacterial proteins to catalyze the recombination reaction. In the case of the Escherichia coli plasmid ColE1, the recombination site, cer, is a 280 bp DNA sequence which is acted on by the products of the argR, pepA, xerC and xerD genes. We have constructed a model system to study this recombination system, using tandemly repeated recombination sites from the plasmids ColE1 and NTP16. These plasmids have allowed us precisely to define the region of strand exchange during site-specific recombination, and to derive a model for cer intramolecular site-specific recombination.
ColE1家族多拷贝质粒所使用的位点特异性重组系统利用两个相同的质粒编码重组位点和四种细菌蛋白来催化重组反应。以大肠杆菌质粒ColE1为例，其重组位点cer是一段280 bp的DNA序列，受argR、pepA、xerC和xerD基因的产物作用。我们构建了一个模型系统来研究这个重组系统，使用来自质粒ColE1和NTP16的串联重复重组位点。这些质粒使我们能够精确界定位点特异性重组过程中的链交换区域，并推导出cer分子内位点特异性重组的模型。
