一种将二维晶体从空气/水界面转移至样品网格的新方法。电子显微镜样品制备/脂质层结晶。

A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization.

作者信息

Asturias F J, Kornberg R D

机构信息

Department of Cell Biology, Stanford University School of Medicine, California 94305, USA.

出版信息

J Struct Biol. 1995 Jan-Feb;114(1):60-6. doi: 10.1006/jsbi.1995.1005.

DOI:10.1006/jsbi.1995.1005

PMID:7772418

Abstract

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.

摘要

本文描述了通过用金属丝环悬浮脂质层上形成的二维（2-D）晶体进行转移的方法。与过去相比，这种方法能更好地回收和保存二维晶体。该方法已应用于酵母RNA聚合酶II的晶体，以便在冷冻水合状态下对其进行分析。