博来霉素诱导的DNA损伤对裂殖酵母粟酒裂殖酵母细胞周期的影响。

Effects of phleomycin-induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle.

作者信息

Belenguer P, Oustrin M L, Tiraby G, Ducommun B

机构信息

Institut de Pharmacologie et de Biologie Structurale, CNRS, Université Paul Sabatier, Toulouse, France.

出版信息

Yeast. 1995 Mar;11(3):225-31. doi: 10.1002/yea.320110305.

DOI:10.1002/yea.320110305

PMID:7785323

Abstract

The effect of phleomycin, a bleomycin-like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe. We report that in response to phleomycin-induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2-phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA-damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.

摘要

博来霉素样抗生素争光霉素对裂殖酵母粟酒裂殖酵母的作用已得到研究。我们报告称，在争光霉素诱导的DNA损伤反应中，粟酒裂殖酵母的生长受到抑制，细胞停滞在细胞周期的G2期。DNA修复突变体rad9和rad17未出现停滞，且对争光霉素高度敏感。未监测DNA复制完成情况就进入有丝分裂的细胞周期突变体对这种DNA损伤剂也表现出更高的敏感性。因此，争光霉素可作为一种工具，用于在粟酒裂殖酵母模型系统中研究DNA损伤和细胞周期检查点，或作为一种新型选择剂。